Plant naNObiology: Nitric oxide metabolism and signaling in responses to nanomaterials
ID: 613 / 257
Proposed Symposium Title: Plant naNObiology: Nitric oxide metabolism and signaling in responses to nanomaterials
Zsuzsanna Kolbert1*, Andrea Rónavári2, Dóra Oláh1, Selahattin Kondak1, Ágota Imre-Deák3, Árpád Molnár1, Réka Szőllősi1, Gábor Galbács4, Zoltán Kónya2
Affiliations: 1 Department of Plant Biology, University of Szeged, Szeged, Hungary 2 Department of Applied and Environmental Chemistry, University of Szeged, Hungary 3 Department of Physical Chemistry and Materials Science, University of Szeged, Hungary 4 Department of Inorganic, Organic and Analytical Chemistry, University of Szeged, Szeged, Hungary
With the development of nano industry, the exposure of plants to nanomaterials increases. As plant signals, nitric oxide (NO) and its reaction products (reactive nitrogen species, RNS) can intensify stress tolerance or participate in the formation of secondary nitro-oxidative stress together with reactive oxygen species. The presentation will summarize our research, which dealt with the effects of zinc oxide nanoparticles (ZnO NPs), nickel oxide NPs (NiO NPs) and multi-walled carbon nanotubes (MWCNTs) of different sizes and concentrations on NO metabolism and signaling. Our experiments revealed that ZnO NPs alters the homeostasis of NO, peroxynitrite, S-nitrosoglutathione (GSNO), hydrogen sulphide, hydrogen peroxide and superoxide radical, and intensify protein nitration and carbonylation, thus overall trigger nitro-oxidative stress, which can contribute to phytotoxicity in Brassica seedlings. Additionally, we demonstrated that the tolerance of Ni hyperaccumulator Odontarrhena lesbiaca ecotypes against NiO NPs manifests at cellular (binding of NPs by the root cell wall due to compositional modification), tissue (root anatomical changes), organ/organism (slight modifications in biomass production) and molecular (changes in RNS metabolism and induced nitrosative protein modification) levels. Our further results suggest that MWCNT triggers secondary nitro-oxidative stress which contributes to its toxicity. Moreover, the results indicate that the extent of the nitro-oxidative processes is associated with the extent of MWCNT toxicity. Overall, different types of nanomaterials induce nitro-oxidative stress in different species and the tolerance against these nanomaterials is associated with NO signaling. Our latest findings on the effect of chitosan-encapsulated GSNO on endogenous NO metabolism and signaling in Brassica seedlings will also be presented.
Acknowledgements: This work was supported by the Hungarian Academy of Sciences (Momentum Programme, LP2023-14).