Format: ORAL

James A. R. Clugston1,2,3, Russell L. Barrett3,4, Matthew A. M. Renner3, Peter H. Weston3, Lyn G. Cook5, Peter C. Jobson4, Brendan J. Lepschi6 and Michael D. Crisp7

1 Hawkesbury Institute for the Environment, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia 2 Montgomery Botanical Center, 11901 Old Cutler Road, Coral Gables, FL 33156–4242, USA 3 National Herbarium of New South Wales, Botanic Gardens of Sydney, Australian Botanic Garden, Locked Bag 6002, Mount Annan, New South Wales 2567, Australia 4 Evolution and Ecology Research Centre, School of Biological, Earth, and Environmental Sciences, University of New South Wales Sydney, Kensington, NSW 2052, Australia 5 School of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, Australia 6 Australian National Herbarium, Centre for Australian National Biodiversity Research, P.O. Box Canberra, ACT 2601, Australia 7 School of Sociology, Research School of Social Sciences, College of Arts and Social Sciences, Australian National University, Canberra 2601, Australia

An Australian endemic clade, Fabaceae tribe Mirbelieae (bush peas and their relatives), currently consist of 27 genera and c. 756 species, and Core Mirbelieae, the focus of our study, contains 21 genera and c. 540 species, or 32% of Australias pea flora. Species within the tribe have a wide distribution across Australia, occupying habitats from coastal to alpine zones, and wet forests to arid deserts. However, the phylogeny of Mirbelieae, required to reach consensus on the taxonomic relationships of species and genera within the tribe, has never been adequately resolved using traditional sequencing. To resolve the phylogeny of Mirbelieae, samples were collected from 354 taxa within the core genera, from a combination of wild collected silica dried leaf tissue or herbarium specimens from NSW, BRI, CANB and MEL. Next generation sequencing was then performed using target capture with the Angiosperms353 universal probe set, with the aim of creating a resolved phylogenetic tree for the group to aid future taxonomy. Data assembly resulted in a total of 278 genes being recovered after filtering, with alignments that ranged from 97 bp to 3,575 bp in length. The resulting phylogenetic tree resolved the backbone of the phylogeny and provided statistical support for all major clades within Mirbelieae. We found that 11 genera were monophyletic and six were polyphyletic, including the largest genus Pultenaea, which was separated into five distinct clades. Ultimately, we published over 30 new species and recognised four new genera. Our findings shed new light on evolution of Mirbelieae and their phylogenetic relationships, providing a new foundation for the future taxonomic research and classification.