Format: ORAL

Miao Sun1*,Xueqin Wang1, Tao Xiong1, Yiying Wang1, XinruZhang1

1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, 430070, China

With the continuous advancement of sequencing strategies, high-throughput sequencing (HTS) technologies have developed as one of the most important tools in plant phylogenomics. Target capture sequencing, particularly hybridization target enrichment sequencing (Hyb-seq), is a crucial HTS sequencing strategy known for its flexible probe design, which has beenbecominga standard method of phylogenomics. The widely used universal Angiosperms353 probe sets (Angiosperms353) play a pivotal role in Hyb-seq and have increased applications in recent years. Wehavecomprehensively assessed the potential application for Angiosperms353 by combing a large amount of literature and empirical datasets.We found that RNA-seq strategy captures the highest number of Angiosperms353 genes, and then followed by whole genome sequencing and genome skimming strategies; increasing sequencing depth leads to higher yields of captured genes; and no obvious bias was observed for the yields of Angiosperms353 across all angiosperm groups we tested. Hence, based on the knowledge acquired above, we used Angiosperms353 extracted from genome resequencing data, and attempted to build phylogeny for all the seed plants, as well as phylogenomics of Elaeagnaceae and Rosaceae. In the era of genomics with large volumes of HTS data, our works demonstrate the practical application of Angiosperms353 by innovatively recycling vast genomic sequencing resources, maximizing genomic data sharing and utilization.