Format: ORAL

Almerekova S., Yermagambetova M., Turuspekov Y., Abugalieva S.

Laboratory of Molecular Genetics, Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan

The Tulipa L. genus belongs to the Liliaceae Juss. family, consisted around 100 species all over the world. Tulipa species are native to southern Europe, North Africa, the Middle East, and Central Asia. The mountainous regions of Central Asia (including Kazakhstan) are considered the primary center of Tulipa origin. According to Ivaschenko (2019) 42 wild Tulipa species are found in Kazakhstan, 18 of them listed in the Red Book of Kazakhstan. These plants have an important economic, floricultural, ecological significances, cultural value in many regions of the World. Wild species are important genetic resources for breeding and play a significant role in ecosystems, especially for pollinators and insects. Ten Tulipa species from Kazakhstan were studied based on complete plastome genome sequences using NovaSeq 6000 (Illumina) platform. All the sequenced Tulipa species from Kazakhstan were deposited to the National Center for Biotechnology Information GenBank and publicly available. Tulipa species plastomes contained 136 genes, including 90 protein-coding genes, 38 transfer RNA, and 8 ribosomal RNA genes (including duplicated genes). The forward, reverse, and palindromic repeat elements and mononucleotide, dinucleotide, trinucleotide, tetranucleotide, pentanucleotide and hexanucleotide repeats were detected in four Tulipa species from Kazakhstan. Furthermore, tandem repeats in each of the Tulipa plastome were identified. Comparative analysis of plastome genome sequences revealed the genes with high polymorphism, which can be used as candidate DNA barcode markers for further studies. This research is funded by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan (Grant No. AP14870612).