Format: ORAL

Thomas L.P. Couvreur1; Carlos Rodrigues-Vaz1,2; Vincent R.C. Soulé1; Laura Holzmeyer1; Francis J. Nge1; Serafin J. R. Streiff1 & Annonaceae Global Phylogenetics Consortium3

1 DIADE, Univ Montpellier, CIRAD, IRD, Montpellier, France 2 Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum National d'Histoire Naturelle-CNRS-SU-EPHE-UA, 57 rue Cuvier, CP 39, 75231 Paris, Cedex 05, France 3 http://www.couvreurlab.org/agpc.html

Annonaceae is a major pantropical family with 2450 known species mainly occurring in rain forests worldwide. Based on decades of taxonomic research and resource generation, Annonaceae provide an excellent model-clade to understand the evolution of hyperdiverse tropical rain forests. I will present ongoing integrative research combining phylogenomics, evolution and ecology of Annonaceae. Using a custom Annonaceae baiting kit, we sequenced hundreds of nuclear genes for over 85% of its diversity worldwide ( 2000 species). Over 75% of species were sequenced from herbarium specimens, the oldest dating from 1785. In parallel, we produced a massive morphological dataset documenting functional diversity, generated precise species-level distribution based on herbarium specimens documenting spatial diversity, and finally, used innovative near infrared scanning (NIRs) to estimate species specific spectra documenting chemical diversity. Most relationships were well resolved producing one of the first ever near-complete robust species level phylogenetic hypotheses of a major tropical rain forest plant family. The results mainly confirmed past systematic studies in Annonaceae, but also produced some surprises. By integrating these large scale datasets with a robust dated phylogenetic tree at the species level, I will present preliminary results about global rain forest diversification. Finally, I will discuss the advantages and challenges of leading a large scale project on a model clade, and how herbaria are (and will be) central to tackling numerous questions about rain forest evolution.