Format: ORAL

Jonas I. Mendez-Reneau1, Erin M. Sigel2

1 Department of Biology, University of Louisiana at Lafayette, 410 East St. Mary Boulevard, Billeaud Hall, Lafayette, LA 70504 U.S.A. 2 Department of Biological Sciences, University of New Hampshire, 105 Main St., Durham, NH 03824 U.S.A

We present a case study using the GoFlag 408 target-capture probe set for ferns to test hypotheses about the progenitors of the allotetraploid fernPolypodium vulgare(Polypodiaceae) across Eurasia. Using our recently published SORTER bioinformatic pipeline, we accurately phased homeologs in 65 allopolyploid individuals, allowing us to resolve different progenitor pairs forP. vulgarein Asia and Europe, as well as three progenitors in the closely allied allohexaploidP. interjectum. We then used by-catch reads to assemble plastid genomes for a subset of samples and generated a time-calibrated phylogeny revealing the maternal progenitors of each polyploid taxon. The results of our combined plastid and nuclear phylogenomic analyses provide support for the segregation of P. okiensein Asia fromP. vulgarein Europe. We highlight the effectiveness of the SORTER pipeline to generate allopolyploid datasets for phylogenetic analyses incorporating a large number of samples at varying ploidy levels.