Format: ORAL

Leandro L. Giacomin1, Juan David Tovar D.2, Tiina Srkinen3,Sandra Knapp4, Thiago Andr5, Clarisse Palma-Silva6, Edeline Gagnon7

1 Universidade Federal da Paraíba, João Pessoa, Brazil 2 Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil 3 Royal Botanic Garden Edinburgh, Edinburgh, UK 4 Natural History Museum, London, UK 5 Universidade de Brasília, Brasília, Brazil 6 Universidade Estadual de Campinas, Campinas, Brazil 7 University of Guelph, Guelph, Canada

Building robust phylogenetic frameworks is the basis to pose any evolutionary question. Nevertheless, understanding relationships among taxa can also benefit society in many other ways, for example, in crop improvement.The economicallyimportant genusSolanum(Solanaceae)hashad significant advances on its systematics in the past decades,whererecent workshaverevealed strong incongruence among phylogenomic datasets and reticulate diversification in deep nodes. We presentgenomicinsights fromtwo unarmed lineages of the genus(clades Brevantherum and Geminata).These lineages together represent 20%ofspecies diversity inSolanum. We generatedtargetcapture data(353 Angiosperm bait set)for 140 speciesand retrieved these regionsfrom publicly available genome skimming data from another 5 species. Our resultsshow that the 353 bait set can be useful to resolve relationshipswithin genera, but more variable regions are desired for recently diversified lineages like Geminata. The heterogeneous length of recovered targetregions seems to influence the support of recovered clades and, as this heterogeneity is related to DNA quality and sample preservation, it might remain as a challenge to any speciose group. Incongruences were found within the 353 bait set alone, and we argue a gene genealogy interrogation method should be employed in these cases. Capturing 353 targeted regions from genome skimming data yielded satisfactory amounts of data for these lineages, and it is a promising approach to compare different datasets and reduce bench work time related to sequence capture. We conclude that universal probe sets such as the 353 can be useful for biggeneralikeSolanum,considering specific baits can be less cost-effective.