Format: ORAL

Rafaela J. Trad1, Flvia F. Pezzini1, R. Toby Pennington1,2, Gwilym Lewis3, Bente Klitgaard3, Russell Barrett4, Anne Bruneau5, Warren Cardinal-McTeague6, Domingos Cardoso 7, James Clugston8,9,10, Manuel de la Estrella11, Ashley N. Egan12, Colin Hughes13, Erik Koenen13, Todd McLay14, Daniel Murphy14, Matt Renner4, Jens Ringelberg15, Rowan Schley2, Mohammad Vatanparast3, Martin Wojciechowski16, Darren Crayn17, Olivier Maurin3, Raquel Negro3, Catherine McGinnie3, Paul Bailey3, William J. Baker3, Flix Forest3, and Gregory J. Kenicer1

1 Royal Botanic Garden Edinburgh, Edinburgh, United Kingdom 2 University of Exeter, Exeter, United Kingdom 3 Royal Botanic Gardens, Kew, Richmond, United Kingdom 4 National Herbarium of New South Wales, Australian Institute of Botanical Science, Mount Annan, Australia 5 Institut de Recherche en Biologie Végétale, University de Montreal, Montreal, Canada 6 Department of Forest and Conservation Sciences, University of British Columbia, Vancouver, Canada 7 Universidade Federal da Bahia, Salvador, Brazil 8 Royal Botanic Garden Sydney, Sydney, Australia 9 Montgomery Botanical Center, Coral Gables, United States of America 10 Hawkesbury Institute for the Environment, Western Sydney University, Penrith, Australia 11 Universidad de Córdoba, Córdoba, Spain 12 Utah Valley University, Orem, United States of America 13 University of Zurich, Zurich, Switzerland 14 Royal Botanic Gardens Victoria, Melbourne, Australia 15 School of Geosciences, University of Edinburgh, Edinburgh, United Kingdom 16 Arizona State University, Tempe, United States of America 17 Australian Tropical Herbarium, James Cook University, Cairns, Australia

Papilionoid legumes occupy every terrestrial biome across a vast array of niches and lifeforms. This charismatic, successful and diverse subfamily (Papilionoideae) is larger than most angiosperm orders and comparable in size to Ericales and Myrtales. The c. 13,800 species constitute around 60% of diversity in the legume family and their value as crops for food, fodder, medicine, soil nitrification, rose wood timber, and an array of other purposes is huge. Due to its size and, until recently, to technical and cost limitations, studies have mostly focused on resolving relationships within specific clades using chloroplast DNA in the subfamily and an extensive nuclear-based phylogeny is still lacking. Additionally, several studies in the most diverse groups of plants have indicated that discordance between nuclear and chloroplast trees is frequent and that phylogenetic estimates for both are necessary to study the evolution of a group. Thus, having a comprehensive nuclear phylogeny for the subfamily is imperative. To this end, we sampled at least one species for over 95% of the 503 papilionoid genera accepted by the Legume Phylogeny Working Group. DNA extraction, library preparation, hybridization with the Angiosperms353 probe set, and sequencing were conducted as part of the Plant and Fungal Trees of Life project at the Royal Botanic Gardens, Kew. We present the first findings of this study, which will contribute to understanding intergeneric relationships and form a basis to enable researchers to investigate many questions across the subfamily on ecology, patterns of diversification, genome duplication. nodulation and character evolution that might help to explain its global success.