Format: ORAL

Rodrigo Mrquez-Sanz 1, Lisa Pokorny 2, Mara Prieto 1, Ana Millanes 1, Isabel Salcedo 3, Sergio Prez Gorjn 4 Ibai Olariaga 1

1 Rey Juan Carlos University, C/Tulipán s/n, 28933 Móstoles, Spain 2 Real Jardín Botánico (RJB-CSIC), Plaza de Murillo 2, 28014 Madrid, Spain 3 University of Salamanca. C/Licenciado Méndez Nieto s/n, 37007 Salamanca, Spain 4 University of the Basque Country (UPV/EHU), Apdo 644, 48080 Bilbao, Spain

The order Cantharellales contains economically important species because of their edibility (Cantharellus, Hydnum), being orchid-mycorrhizal (Tulasnella) or plant pathogens (Rhizoctonia). However, within this group nuclear ribosomal regions produce phylogenetic conflicts due to an accelerated rate of evolution and cause long-branch attraction problems, making them useless to infer phylogenetic relationships between family clades. This is why, despite 4 monophyletic lineages Hydnaceae, Cejpomycetaceae, Tulasnellaceae, Botryobasidiaceae are consistently retrieved in phylogenetic studies, their relationships remain unclear and are strongly dependent upon the genetic marker and taxon sampling employed. In this scenario, we designed a kit to capture 526 orthologous single-copy protein-coding genes in Cantharellales filtered from 10 representative genomes. Filtered genes were sequenced across a set of more than a hundred samples of Cantharellales, representative of its diversity and including many generic types. The analysis of the dataset containing DNA sequences of 526 genes resulted in a fully supported backbone for the Cantharellales, with relationships among the Ceppomycetaceae, Tulasnellaceae, Botryobasidiaceae and Hydnaceae resolved. Novel phylogenetic relationships are revealed within the Hydnaceae. Our results show that an updated classification at generic level needs to be proposed urgently within the Cantharellales. We infer from our test that Cantharellales-kit constitutes an excellent approach to construct a multiple-marker, fully supported phylogeny of Cantharellales. Our test suggests furthermore that the kit will function well in sister groups of Cantharellales, such as in Dacrymycetales and Sebacinales.