Format: ORAL

R. Shawn Abrahams1, Shawn Thomas2, Tatiana Arias, Jacob Washburn2, J. Chris Pires

1 Department of Ecology & Evolutionary Biology, Yale University, New Haven, CT 06511, USA. Department of Biochemistry, Purdue University, West Lafayette, IN 47907-2063. r.shawnabrahams@gmail.com, ORCID ID: 0000-0003-1749-2040 2 Division of Biological Sciences and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211-7310, USA

The Brassiceae tribe of the Mustard Family has a complex evolutionary history of whole genome duplication and hybridization. These mechanisms contribute to phenotypic plasticity and adaptability of the tribe, while also confounding attempts to understand the relationships among the species of the tribe. To account for these complications, we utilize a genome-guided phylotranscriptomic method that leverages genomic synteny to inform phylogenomic inference. With this method, we infer a nuclear tree with a novel topology that places the Crambe clade as sister to both the Nigra and Rapa/Oleracea group species. Further investigation of single copy genes from the ancestral sub-genome of the tribal hexaploidy identified differences in topology among genes derived from different parental genomes. These findings help explain why we see various topologies based on the data type method of inference when attempting to reconstruct clade relationships.