Format: ORAL

Chun-Xia Zeng1, Jun-Bo Yang1

Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming Yunnan 650201, China;

In Pharmacopoeia of the Peoples Republic of China 2020th edition, Mori Folium,Mori Cortex, Mori Ramulus,Mori Fructus,however,botanical origin of all of thesefour medicines are limited to only one species, M. alba. The Japanese Pharmacopoeia Eighteenth Editionalso limit the botanical origin of Mori Cortex to M. alba.Because of polyploidization and hybridization occured frequently in Morus,we found that not a few individuals and specimens of Morus plants are difficult to identify because of their intermediate morphological characteristics between species especially M. alba and other species.For the safety and standardization of medication, it is particularly necessary to answerthat the mecinal plant Morus albais really a goodspecies or not? The previous phylogentic analysesof ITS and cpDNA have been reported most of the Morusspeices collected in China were grouped into a clade lacking for phylogentic resoulition. Genome skimming has the potential for generating large data sets for DNA barcoding and wider biodiversity genomic studies, particularly via the assembly and annotation of full chloroplast (cpDNA)?nuclear ribosomal DNA (nrDNA) sequencesand single copy orthologs (SCOs).Based on phylogenetic analyses of plastome sequences and ITS sequences, most Morus species grouped into a large clade lacking for phylogenetic resolution. Except for M. notabilis, other 8 species all should be considered as the botanical origin od Mori Cortex. However, based on 272 SCOs, Morus albacould be a monophyletic species. Plant species identification is difficult because of the main reasons being incomplete lineage sorting, artificial cultivation, natural hybridization and chloroplast capture. It appears that nuclear genome data have a vital role in species identification and are expected to be used as next-generation nuclear barcodes.