Format: ORAL

Lizzie Roeble1,2, Marina Ventayol Garca1, Claudia Baider3, Vincent Florens4, Matthew Knope5, Joshua Lambert2, David Lorence6, ??Cliff Morden7, Dominique Strasberg8, Christophe Thbaud9, Kenneth Wood6, Rampal S. Etienne2, Luis Valente1,2

1 Naturalis Biodiversity Center, Darwinweg 2, 2333 CR Leiden, The Netherlands 2 Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, The Netherlands 3 The Mauritius Herbarium, Agricultural Services, Ministry of Agro-Industry and Food Security, Reduit, 80835, Mauritius 4 Tropical Island Biodiversity, Ecology and Conservation Pole of Research, Faculty of Science, University of Mauritius, Le Réduit, Mauritius 5 University of Hawai?i at Hilo, Dept. of Biology, 200 W. Kawili St., Hilo, HI 96720, USA 6 National Tropical Botanical Garden, Kalaheo, Hawaii, USA 7 School of Life Sciences, University of Hawai?i at Manoa, Honolulu, Hawai?i, USA 8 Peuplements Végétaux et Bioagres-seurs en Milieu Tropical, UMR PVBMT, Université de La Réunion, Saint-Denis, La Réunion, France 9 Laboratoire Évolution and Diversité Biologique (UMR 5174), CNRS- IRD- Université Paul Sabatier (Toulouse 3), Toulouse, France

Macroevolutionary models of island biogeography applied to high-throughput DNA sequencing data on island species are promising tools for inferring the processes governing biota assembly for entire communities over evolutionary time scales. However, to date, phylogenetic trees of island plant lineages that can be used for studying island biogeography have mostly been reconstructed using a limited number of loci, often leading to poor resolution, particularly in rapidly evolving insular radiations. Asteraceae - the largest plant family in the world - provides an excellent model system for looking at diversity dynamics on islands, due to this groups high diversity, endemism, and number of radiations across islands globally. In particular, the Asteraceae floras of two remote oceanic archipelagos - Hawai?i and the Mascarenes - are remarkably diverse. The Hawaiian Islands have 99 native Asteraceae species of which 98 are endemic, and are home to the iconic silversword alliance, a textbook example of adaptive radiation. The Mascarenes also have a high proportion of Asteraceae endemism (89%), but until now most of the native Asteraceae lineages on the Mascarenes have not been studied in a phylogenetic context. Here, we compile and build new dated phylogenies with target capture sequencing (Hyb-Seq) using both the Angiosperm-353 and the Compositae-1061 kits for all the native Asteraceae lineages that have colonized these two isolated oceanic archipelagos. We then use a macroevolutionary island biogeography model (DAISIE) to analyze and reconstruct the build-up of the diversity for the entire native Asteraceae assemblage on Hawai?i and the Mascarenes. Our approach using target-capture sequencing allows us to infer the number of colonization and radiation events with increased precision, estimate macroevolutionary rates of colonization, speciation and extinction, and to test whether these systems are at equilibrium.