Format: ORAL

Matti A. Niissalo1, Sitaram Rajaraman2,3, Jun Ying Lim2,4, Le Min Choo1, Jia Jun Ngiam2, Kang Min Ngo5, Shawn K. Y. Lum5, Victor A. Albert2,6, Charlotte Lindqvist2,6, David J. Middleton1,2, Gillian S. Khew1,2, Jarkko Salojrvi2,3

1 Singapore Botanic Gardens, National Parks Board, Singapore 2 School of Biological Sciences, Nanyang Technological University, Singapore 3 Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland 4 Department of Biological Sciences, National University of Singapore, Singapore 5 Asian School of the Environment, Nanyang Technological University, Singapore 6 Department of Biological Sciences, University at Buffalo, New York, USA

Approximately half of Singapores native plant diversity resides in a small, 1.64 km2 patch of rainforest in Bukit Timah Nature Reserve (BTNR). Between 2019 to 2022, we collected and vouchered a total of 882 angiosperm species from BTNR. Illumina shotgun short-read sequencing was carried out for each species to obtain a minimum of 30Gb of data, followed by genome assembly and gene prediction. For the first stage of the project we focused our analysis on 499 good quality genomes. A set of 1,000 conserved single-copy genes were used to generate a high-resolution phylogeny of the BTNR species, illustrating that when compared with APG IV, there were differences in the position of Santalales and the relationships within the families in Malpighiales and some orders in the malvids and fabids. Gene predictions allowed us also to study ancestral whole genome duplication events, to look for evidence of introgression and to chart the proteomic diversity present in BTNR.